Anti-depression effect of Xiao-yao-san series prescriptions with plasma NMR metabolomics
会议名称：《中国药理学会第十一次全国学术会议》
会议日期：2011年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：The project supported by National Natural Science Foundation of China(30772759) National Natural Science Foundation of China(30901960) National Natural Science Foundation of China(81001688) Programs of International Science and Technology Cooperation of China(2008DFA30430)
关 键 词：Xiaoyaosan series prescriptions depression metabonomics NMR mechanism
摘 要：OBJECTIVE To discriminate the anti-depression effect of Xiao-yao-san series prescriptions on forced swimming test (FST) rat model with *** The forced swimming test rat model was used to evaluate the action of antidepressant and the rat plasma was collected ***-yao-san(XY),Dan-zhi-xiao-yao-san(DZ) and Chai-hu-shu-gan-san (CH) formula were extracted with aqueous solution,and the final concentration was 46.3,48.9 and 7.9 g·kg(the total amount of crude drug),respectively.54 rats were divided into 6 groups *** saline,velafaxin and Xiao-yao-san series extractions were orally treated to the rat groups for 2 weeks.A metabonomics method was used to evaluate the pathogenesis of depression and the mechanism of Xiao-yao-san *** samples were prepared and measured using NMR,and the data were processed with Microsoft Excel or analyzed by SIMCA-P *** importance in projection statistics and loading plot were used to screen relative *** Significant difference was observed between the FST group and control group,which was an illustration that the pharmacological model was duplicated *** effect of Xiao-yao-san was similar to that of positive drug venlafaxin,while the effect of Dan-zhi-xiao-yao-san and Chai-hu-shu-gan-san is not as good as *** with the control group,significant changes were observed in lactate,thrronine,Glu,choline,glucose in serum of positive and Xiao-yao-san *** The NMR analysis suggested that Xiao-yao-san prescription was of significant anti-depression effect which is better than the effect of Dan-zhi-xiao-yao-san and *** forced swimming test rat model is a mature model to evaluate the pathogenesis of depression and to discriminate the anti-depression effect of Xiao-yao-san series prescriptions.