A Monoclonal Antibody-based Sensitive Enzyme-linked Immunosorbent Assay(ELISA) for the Analysis of Three Organophosphorous Pesticides in Real Samples
会议名称：《中国植物病理学会第九届全国会员代表大会暨2010年学术年会》
会议日期：2010年
学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护]
摘 要：According to the common structure of three organophosphorus pesticides (Isocarbophos,Isofenphos-methyl and Isofenphos),hapten(H1) was synthesized. Bovine serum albumin(BSA) and ovalbumin(OVA) were used as protein carriers respectively to couple with H1 by an active ester *** antigens H1-BSA and H1-OVA were prepared as immunogen and coating antigen,*** last, one cell strain of monoclonal antibody was obtained and named 6B5/E3,which can be used to detect the three *** optimization,the standard curves of these three pesticides were established and the detection limits of the three pesticides were 12.19,34.51 and 19.68 ng/ml,*** addition,other six kinds of organophosphorus pesticides were tested by using this assay and the results were regarded as *** recoveries obtained by the three standard pesticides addition to real samples,including rice,water and soil,were all from 91.63%to 112.86%.Therefore,the optimized ELISA might become a convenient and satisfied analytical tool for monitoring Isocarbophos,Isofenphos-methyl and Isofenphos residues in real samples.