Identification of novel cell proliferation-enhancing genes by random siRNA library screening
作者单位：Institute of Basic Medical SciencesChinese Academy of Medical Sciences(CAMS)/ School of Basic MedicinePeking Union Medical College(PUMC)National Laboratory of Medical Molecular Biology Institute of Molecular MedicinePeking University
会议名称：《中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议》
会议日期：2010年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
摘 要：RNA interference(RNAi) has become a powerful research tool in functional genomics *** have previously reported the construction of a random small interfering RNA (siRNA) *** library is versatile as it avoids siRNA sequence bias,and targets both known and unknown genes. Here we report a high throughput screening with this random siRNA library to reveal novel genes that can accelerate preosteoblast MC3T3-E1 cell *** siRNAs that enhance MC3T3-E1 cell proliferation were identified.