NF-κB activation protects hippocampal neurons from oxidative damage induced by prenatal stress in offspring rats

作     者：Ning JIA~(1,2),Liang SONG~3,Hui LI~4,Qin-Ru SUN~2,Xiao-Li FAN~1,Zhong-Liang ZHU~(5,*) 1 Department of Physiology and Pathophysiology,School of Medicine,Xi’an Jiaotong University,Xi’an 710061,China 2 Department of Human Anatomy,Histology and Embryology,School of Medicine,Xi ’an Jiaotong University,Xi ’an 710061,China 3 Shaanxi University of Chinese Medicine,Xianyang 712046,China 4 Department of Pediatrics,First Affiliated Hospital of Medical College,Xi’an Jiaotong University,Xi’an 710061,China 5 Department of Pharmacology,School of Medicine,Xi’an Jiaotong University,Xi’an 710061, China 

会议名称：《中国生理学会第23届全国会员代表大会暨生理学学术大会》

会议日期：2010年

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(30670672) 

关 键 词：NF-κB prenatal stress oxidative damage mitochondria offspring hippocampus 

摘      要：Prenatal stress(PS) has been shown to injure hippocampal neurons,but the mechanism of neuron injury remains *** the present study,to investigate the role of NF-κB in the oxidative damage of hippocampal pyramidal neurons induced by PS,we observed the neuronal ultrastructure,examined the inner mitochondrial membrane potential(EMMP),determined the translocation of NF-kB to the nucleus and the binding activity of NF-kB to DNA,and measured the transcription of its target genes(Mn-SOD and CaBP-D28k) in *** rats were divided into two groups:control group(CON) and prenatal stress group(PS).Mothers of the CON group were left undisturbed throughout the *** of the PS group(late gestation,days 14-20 of pregnancy) were exposed to restraint stress three times a day,and each time lasted 45 *** offspring rats were used at *** neuronal ultrastructure of hippocampal CA3 region in PS offspring appeared bulgy mitochondria with unclear membrane and irregular electron density and more lipofuscin *** IMMP of hippocampal neurons was significantly less in PS offspring than that in CON(P0.01).PS offspring had more expression of nucleus NF-kB in hippocampus compared to CON(P0.001).The binding activity of NF-kB to DNA was significantly higher in PS offspring than that in CON(P0.01).PS resulted in a significant induction of Mn-SOD mRNA and CaBP-D28k mRNA in hippocampus of offspring(P0.01).Taken together,we concluded that NF-kB pathway is activated by ROS,although excessive ROS induced by PS can cause oxidative damage to *** activation of NF-kB and the increase of Mn-SOD mRNA and CaBP-D28k mRNA expression might contribute to reduce intracellular ROS and Ca and attenuate hippocampal neurons oxidative damage induced by PS.