Identification of tumor stem-like cells in human multiple myeloma cell lines
作者单位：Department of Pathogenic Biology and Immunology Medical School Southeast University
会议名称：《第八届全国免疫学学术大会》
会议日期：2012年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：Human multiple myeloma Tumor stem cells identification Targeting therapy
摘 要：Background:Multiple myeloma(MM) is a malignancy of terminally differentiated plasma cells that are predominantly localized in bone *** still remains incurable in most of the *** hypothesis of tumor stem cells(TSCs) states that although chemotherapy kills the malignant plasma cells, it is believed to leave a reservoir of TSCs that continue *** to identify TSCs in MM cell lines, however, remains to further ***:The purpose of this study was to isolate and identify tumor stem-like cells from MM cell lines RPMI-8226 and NCI-H929 and provided an experiment data for targeting therapy of MM TSCs in ***:The mouse antihuman CD138 and CD34 antibodies coupled to magnetic microbeads were respectively used to isolate CD138-CD34-cells from the RPMI-8226 and NCI-H929 cell lines by magnetic associated cell *** characteristics of CD138- CD34- cells were analyzed by the colony formation assay in the soft agar media, proliferative assay, drug resistant assay, transwell assay and the tumorigenic assay in nonobese diabetic/severe combined immunodeficiency(NOD/SCID) mice, ***:The isolated CD138-CD34-cells were capable of forming colonies in the soft agar media, exhibited high proliferative and migratory potency, possesed a strong drug resistant to paclitaxel and had a powerful tumorigenicity in NOD/SCID mice compared with non-CD138-CD34-MM ***, CD138-CD34- cells were found to possess the characteristics of tumor stem-like cells, such as high proliferative potency, more self-renewal and stronger ***:The biological characteristics of CD138-CD34-cells suggested the cells might be tumor stemlike cells in MM cell lines RPMI-8226 and *** data contributes to the identification of molecular targets important for future MM TSC targeting therapy.