Up-regulation of HO-1 via Akt/Nrf2 pathway by Notoginsenoside R1 rescues PC12 cells from oxidative stress
作者单位：Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal MedicineMinistry of EducationInstitute of Medicinal Plant DevelopmentChinese Academy of Medical Sciences & Peking Union Medical College Medical SchoolNanjing University
会议名称：《2013年全国老年性痴呆与相关疾病学术会议》
会议日期：2013年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
摘 要：Object:To study the neuroprotective effects of Notoginsenoside Rl(NGRl) against HO-induced oxidative stress in PCI2 cells and the underlying mechanism. Results:In this study,for the first time,we clearly showed protective effects of NGRl against HO-induced oxidative stress in PC 12 ***(10 and 100μM) pretreatment obviously inhibited HO-induced ROS accumulation and oxidative stress in *** pretreatment could also suppress HO-induced the imbalance of Bcl-2 family protein,disruption of mitochondrial membrane potential and activation of caspase-3 as well as inactivation of Akt/Nrf2 ***,we fatherly clarified a novel underlying *** treatment alone could up-regulate HO-1 in dose- and time- dependent manners,and NGRl-mediated up-regulation of HO-1 was abolished by transfection with Nrf2 siRNA and supplementation with a PI3K specific inhibitor *** treatment alone could also induce phosphorylation of Akt,facilitate the nuclear accumulation of Nrf2,and enhance the binding activity of Nrf2-ARE in dose- and time- dependent *** importantly, NGRl-mediated activation of Nrf2/ARE patliway was suppressed by transfection with Akt siRNA and ***,NGRl-mediated protection was also attenuated by a HO-1 specific inhibitor Snpp and LY294002. Conclusion:NGRl provides neuroprotective effects against oxidative stress in PC 12 cells by up-regulation of HO-1 through Akt/Nrf2 pathway.