Salvianolic acid B protects endothelial cells from oxidant-mediated damage
会议名称：《两岸三地药理学与临床药理学术会议》
会议日期：2008年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
摘 要：Objective To investigate the protective effects of Salvianolic acid B(Sal B)on hydrogen peroxide (HO)-induced injury in human umbilical vein endothelial cells(HUVECs).Sal B is considered as one of the most active anti-oxidant and the major pharmacological component of the herb,Salvia *** beneficial effects include hepatoprotection,elicitation of endothelium-dependent vasodilation,lowering blood pressure in hypertension,inhibition of HIV-1 replication and suppressing inflammatory cytokine-stimulated endothelial adhesiveness to human monocytic cells by its strong antioxidant *** Treatment with HO significantly decreased the cell viability and increased the lactate dehydrogenase(LDH)leakage that is an apoptotic *** with Sal B prevented significantly from HO-induced cell apopto- sis and other damages in a concentration-dependent *** mechanism of Sal B protection was studied with two-dimensional gel electrophoresis(2-DE)coupled to hybrid quadrupole time-of-flight mass spec- trometry(Q-TOF)mass *** Data base searching implicated glucose-regulated protein 78 (GRP78),a central regulator for ER stress,was up-regulated in Sal B-exposed *** exposure to Sal B,the level of activating transcription factor 4(ATF4)was raised,with a transient phosphorylation of the a subunit of eukaryotic translation initiation factor(eIF2α).Knock-down of GRP78 by siRNA signifi- cantly reduced protective effects of Sal *** These results suggest that Sal B-induced GRP78 up- regulation via phosphorylation of eIF2αand resultant translation of *** up-regulation of ER chaper- ones induced by Sal B may play an important role in protecting human endothelial cells from oxidative stress-induced cellular damage.