Sio3 is the channel mediating KSper potassium current in sperm
会议名称：《中国药理学会第七届全国生殖药理学术交流会》
会议日期：2011年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
摘 要：Slo3 channel is the primary candidate mediating the pH-dependent K current (KSper) in mouse ***,because heterologous expression of Slo3 results in currents that are much less activated at physiological conditions that are native KSper currents,the equivalence of Slo3-dependent current and KSper remains ***, we show that genetic deletion of Slo3 abolishes all pH-dependent K current at physiological membrane potentials in corpus epididymal *** potentials above 0 mV, a residual pH-dependent outward current(I) is observed in Slo3-/-*** inhibition of KSper/Slo3 and I by clofilium reveals that the amplitude of I is similar in both wt and Slo3-/-sperm,strongly supporting the idea that I is an intrinsic component in native *** properties of I suggest that it likely represents outward monovalent cation flux through CatSper ***/Slo3 may account for essentially all mouse sperm K current and is the sole pH-dependent K conductance in these *** deletion of *** sperm exhibit deficits from distinct aspects, including membrane potential response to *** in the absence of bicarbonate,and morphological adaption under hypotonic *** a consequence, Slo3-/-male mice are *** these results show that KSper/Slo3 is the primary spermatozoan K current which is critical for normal sperm *** results also suggest that Slo3 channel is a potential target for developing male contraceptives.