Overexpression of the transcription factor Sp8 induces ectopic motor neurons in the p3 domain of chick spinal cord
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：Sp8 motor neuron Nkx2.2 dorsoventral patterning spinal cord
摘 要：正During the period of neurogenesis in the spinal cord,particular types of neurons are generated from specific progenitor cells located in different dorsoventral（DV） regions of the ventricular *** graded morphogens along the DV axis of the neural tube confer positional information to the progenitor *** achieve through induction or inhibition of a set of transcription factors expression in a concentration dependent *** combinatorial expression of distinct transcription factors,the DV axis of the neural tube can be divided into multiple domains and cross-repressive interactions between complementary pairs of transcription factors further refine and sharpen the boundaries between adjacent *** action of these transcription factors directs different progenitor populations to generate particular subtypes of ***8 is a zinc finger transcription factors belonging to specificity protein（Sp） family and expression of Sp8 in the basal plate of spinal cord has been *** there is no study addressing the function role of Sp8 during spinal cord *** the present study,we first confirmed Sp8 expression in the spinal cord using *** neurogenesis in the spinal cord,Sp8 expression shares the same ventral limit with O1ig2 and is complementary to Nkx2.2.O1ig2+ domain and Nkx2.2+ domain are neighboring with each other,and give rise to Isll+ motor neurons and Siml+ V3 interneurons respectively. When mouse Sp8 was overexpressed in the chick spinal cord using either retrovirus or in ovo electroporation,we found that ectopic motor neurons were induced in the Nkx2.2+ p3 ***,Nkx2.2 expression was cellautonomously repressed by Sp8 *** suggests that Sp8 participates in patterning the ventral spinal cord.