Protective role of Vδ2 γδ T cells in patients with chronic hepatitis B through regulating production of the Th17 cells
作者单位：Colege of Life Sciences Nankai University Research Center for Biological Therapy Institute of Translational Hepatology Beijing 302 Hospital
会议名称：《第八届全国免疫学学术大会》
会议日期：2012年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
关 键 词：Liver damage Immune regulation Chemotaxis Proliferation Immune therapy
摘 要：背景:Chronic hepatitis B continues to be a major health problem globally with disease progression from liver inflammation to cirrhosis and hepatocellular *** innate and adaptive immune cells involved in the liver ***, γδ T cells, especially Vδ2 T cells role in chronic hepatitis B virus infection remain unclear.目的:To determine the character of Vd2 T cells in CHB patients and the regulate role of them in CHB patients 方法:Here we characterized peripheral and intrahepatic γδ T cells, and analyzed their association with liver injury in a cohort of HBV-infected patients, including 64 immune-activated(IA) patients, 22 immune-tolerant(IT) carriers and 30 healthy controls(HC).To achieving this, flow cytometry was adopted.结果:We demonstrated that the number and percentage of peripheral and hepatic Vδ2 T cells were significantly lower in IA patients than HC and IT subjects, and the number of Vδ2 T cells was inversely correlated with liver injury, but not HBV DNA load.Vδ2 T cells in IA patients exhibited an impaired proliferative capacity due to abnormalities in the G2/M cell cycle phases, and a skewed chemotactic ability contributing to the lower number of these cells in ***, an in vitro co-culture assay showed that Vδ2 T cells significantly suppressed the production of the Th17-associated cytokines, interleukin(IL)-17 and IL-22, by CD4+ T cells in both cell contact-dependent and IFN-γ-dependent mechanisms.结论:The infection microenvironment in IA patients results in decreased numbers of Vδ2 T cells in peripheral blood and liver, which in turn impairs the suppression of Th17 cell function and reduces liver ***, adoptive transfer of Vδ2 T cells may provide a novel therapeutic approach for IA patients.