Pyrosequencing analysis of early childhood caries from Chinese She and Han race
作者单位：Department of Conservative Dentistry and PeriodonticsAffiliated Hospital of StomatologyCollege of MedicineZhejiang University State Key Laboratory for Diagnosis and Treatment of Infectious Diseasesthe First Affiliated HospitalCollege of MedicineZhejiang University
会议名称：《2014年第九次全国牙体牙髓病学学术会议》
会议日期：2014年
学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学]
关 键 词：Dental caries Microbiota Plaque
摘 要：Objective:Dental caries is the most common chronic childhood disease in the ***,there has been few knowledge about the oral microbiota of childhood from different *** aim of the study is to explore the microbiota in dental plaque from Chinese Han and She children by pyrosequencing ***:Supragingival plaque were collected from 66 She and 60 Han children(3- 6 years old)with or without dental *** V1-V3 hypervariable regions of bacterial 16 S rDNA genes were amplified by multiply primers and analyzed by 454 high-throughput ***:A total of 716,972 reads passed the quality control,belonging to eighteen phyla and 102 *** genera including Actinomyces,Corynebacterium,Derxia,Leptotrichia,Neisseria,Streptococcus,Veillonella,Capnocytophaga,Prevotella,Granulicatella,Fusobacterium,Johnsonella,Kingella,Porphyromonas,Abiotrophia were shared by 97%She and Han children with or without dental *** genera constituted 86.2-88.9%of the total microbiota of dental plaque in all *** the quantity of dental plaque microbiota composition from She and Han subjects were *** genera of that such as Actinomyces,Campylobacter,Neisseria,Leptotrichia,Prevotella,Porphyromonas,Capnocytophaga showed significantly different between the Han and She *** three genera including Gemella,Bergeyella,Granulicatella were associated with dental caries in She group,whereas other nine genera including Streptococcus,Actinomyces were associated with dental caries in Han ***:The framework of normal plaque microbiota from She and Han race was *** microbiota disparities are present in different racial groups and the potential cariogenic microbes in She and Han children was at a certain extent variable.