The Subcellular Localization of Six Proteins of Rice stripe virus in Nicotiana benthamiana
会议名称：《中国植物病理学会第九届全国会员代表大会暨2010年学术年会》
会议日期：2010年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Rice stripe virus(RSV) Localization N.benthamiana
摘 要：Rice stripe virus(RSV) is the type member of the genus *** genome of RSV is composed of four negative-sense single-stranded RNA segments,designated RNA 1,2,3 and *** 1 is of negative polarity,encoding the putative RNA dependent RNA polymerase. The other three segments adopt an unusual ambisense coding strategies *** the viral-sense RNA and viral complementary-sense RNA possess coding *** determine the subcellular localizations of proteins of RSV(without RdRp),we constructed six ORFs of RSV(NS2,NSvc2, NS3,CP,NSvc4,and SP) in pEarleyGate 101 and pEarleyGate 104 vectors by Gateway technique to yield N-terminal or C-terminal YFP fusion *** subcellular localization of six proteins of RSV fusion with YFP was observed by confocal microscopy at 2 days after agroinfiltration in the leaves of Nicotiana benthamiana,*** found the NS2-YFP formed punctate structures at the periphery of cell or within nucleus in *** *** microscopy confirmed that NS2 of RSV distributed along cell wall in RSV-infected wheat after labeling by RSV NS2 *** membrane protein NSvc2-YFP formed distinct vesicles throughout the ***3-YFP and SP-YFP were diffuse distributing in cytoplasm and *** transient expression of CP-YFP led to formation punctate structures around *** NSvc4-YFP led to formation punctate structures targeting to plasmodesmata(PD), corresponding to its MP function.