Neuroprotective effects of ginsenoside Rgl on the dopaminergic neuronsin the substantia nigra of MPTP-treated C57BL6 mice
会议名称：《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》
会议日期：2007年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Parkinson’s disease Ginsenoside Rg1 1-methy-4-pheny1-1,2,3,6-tetrahydropyridine(MPTP) dopamine apoptosis iron
摘 要：正In the present study,the effects of Ginsenoside Rgl（Rgl） on l-methy-4-phenyl-l,2,3,6-tetrahydropyridine （MPTP） induced dopamineregic neuron degeneration in the substantia nigra（SN） of C57BL6 mice were *** high-performance liquid chromatography electrochemical detection,we showed that the dopamine content in the striatum decreased after MPTP treatment,which could be restored by *** immunohistochemistry and semi-quantitative RT-PCR, we showed that Rgl could prevent the MPTP-induced decrease of tyrosine hydroxylase（TH） positive neurons and the TH mRNA expression in the substantia nigra（SN）.Further experiments demonstrated that Rg1 could reverse the changes of bax immunoreactive cells and the bax mRNA expression,bcl-2 immunoreactive cells and the bcl-2 mRNA expression as well as caspase-3 immunoreactive cells and the caspase-3 mRNA expression in the SN of MPTP treated mice. By Perls’ iron staining study we demonstrated that Rg1 prevented the MPTP-induced increase of iron-staining cells in the *** results suggested that the protective effects of Rg1 on the toxicity of MPTP could be attributed to its anti-apoptotic and iron- chelator-like properties.