Hydrogen peroxide-mediated activation of mitogen-activated protein kinase 6 modulates nitric oxide biosynthesis and signal transduction in Arabidopsis thaliana

作     者：Pengcheng Wang~1,Yanyan Du~1,Yuan Li~2,Dongtao Ren~2,Chun-Peng Song~(1*) 1 Laboratory of Plant Stress Biology,Department of Biology,Henan University,Kaifeng 475001 2 State Key Laboratory of Plant Physiology and Biochemistry,China Agricultural University,Beijing 100193 

会议名称：《中国植物学会植物细胞生物学2010年学术年会》

会议日期：2010年

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

摘      要：Nitric oxide(NO) is a bioactive molecule that functions in numerous physiological and developmental processes in plants,including lateral root *** this study,we used biochemical and genetic approaches to analyze the function of Arabidopsis mitogen-activated protein kinase 6(MPK6) in the regulation of NO synthesis in response to hydrogen peroxide(HO) during lateral root *** both mpk6 mutants studied,HO-induced NO synthesis and nitrate reductase(NR) activity were decreased ***,the NR isoform,NIA2,was required for the MPK6-mediated production of NO induced by ***,NIA2 interacted physically with MPK6 in vitro and in vivo,and also served as a substrate of *** 627 was the putative phosphorylation site of *** of NIA2 by MPK6 led to an increase in NR activity. Phenotypical analysis revealed that mpk6-2 and mpk6-3 seedlings produce more and longer lateral roots than that wild-type plants did after application of the NO donor sodium nitroprosside(SNP) or HO. These data support strongly a novel function of MPK6 in modulating NO production and signal transduction in response to HO during root development.