Hsp90αinvolves in cell damages and apoptosis after heat stress in primary myocardial cells of neonatal rat
作者单位：College of Veterinary MedicineNanjing Agricultural University
会议名称：《2012年中国畜牧兽医学会兽医病理学分会暨中国病理生理学会动物病理生理专业委员会学术研讨会》
会议日期：2012年
学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学]
摘 要：This study investigated the effect of heat stress on primary myocardial cell damage,histopathological lesions,apoptosis and it’s signal transduction molecules and the expression of Hsp90αprotein and its coraresponding mRNA in response to heat stress in *** inc ubated at 37℃for 72 h in humidified atmosphere of 5%CO and 95%air,the myocardial cells were suddenly heat stressed for 10 min,20 min,40 min,1 h,2 h,4 h,6 h and 8 h in another incubator with 95%air and 5%CO at 42℃.Levels of CK enzyme,histopathology,apoptosis,apoptosis signal transduction and hsp90αwere detected using enzymatic assay,histopathological technique,flow cytometry and western blot *** levels were gradually changed during heat stress,suggesting that the myocardial cells were distinctly stressed and obviously damaged by the heat *** results of flow cytometry showed that apoptotic levels at 40 min and 8 h of heat stress were significantly higher than those of the control(P0.01 and P0.05) respectively. Levels of cytochrome c and cleaved caspase-3 significantly increased at 40 min heat *** 40 min heat stress,hsp90αshow significant *** results demonstrate that apoptosis inhibit hsp90α.In contrast,Hsp90αsignificantly increased(P0.01) at 6 and 8 h heat *** elevation of hsp90αin the primary myocardial cells is important marker at the later time of the heat shock and may act as a protective protein.