Erythromycin Resistance Study by Label-free Quantitative Proteomics Analysis in Methicillin-resistance Staphylococcus aureus
作者单位：Department of Chemical and Biomolecular Engineeringthe Hong Kong University of Science and Technology China State Institute of Pharmaceutical IndustryShanghai Institute of Pharmaceutical Industry Division of Biomedical Engineeringthe Hong Kong University of Science and Technology
会议名称：《第十二届全国抗生素学术会议》
会议日期：2013年
学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 10[医学]
基 金：supported by the University Grant Council of the Hong Kong Special Administrative Region Government,China(Grant No.HKUST DAG08/09.EG02) the National Natural Science Foundation of China(Grant No.81273413)
关 键 词：Erythromycin,Methicillin-resistanceStaphylococcus aureus label-free quantitativeproteomics
摘 要：Background: Staphylococcus aureusis a virulent pathogen that is commonly found in hospitals and community ***-resistance ***(MRSA),which has developed resistance to almost all kinds of antibiotics,is a pathogen that is responsible for over 50%*** worldwide and the mortality rate of MRSA infection is more than 20%.Due to its multi-drug resistance,it is challenging to control and treat this pathogen with standard antibiotic *** has been considered the last-resort treatment;however,even vancomycin-resistant *** has been reported ***, there is an urgent need to develop new antibiotic drugs or new strategies ***,a label-free quantitative proteomics method has been developed to study the MRSA resistance study to oxacillin in our *** method not only recovered the main resistance mechanism to oxacillin-treated MRSA,but also provided a system-wide view of how MRSA respondto *** this paper,erythromycinresistance mechanism is studied by the same label-free quantitative proteomics method. Methods A sub-lethal dose of erythromycin was applied to MRSA strain ATCC 43300,which is resistance to *** control group was ATCC 43300 cultured without antibiotic *** bacteria were cultured in Muller Hinton brothand grew to OD *** cells were collected and lysed by sonicationin 8 M *** were precipitated by cold *** shotgun proteomics techniques were applied to prepare and analyze the samples on a Thermo Scientific LTQ Velos *** biological replicates were performed for the erythromycin treated and control *** technical replicates were prepared and analyzed for each biological replicate to validate the reproducibility of the sample preparation and the performance of the LC-MS/MS instrument.A customized Staphylococcus aureus protein database was constructed from 23Staphylococcus aureusstrains with complete proteome(redundan