Effect of mlnc RNA2Y on the neuropathic pain mediated by P2X3 receptor in dorsal root gangli-on
作者单位：Department of PhysiologyMedical College of Nanchang University
会议名称：《中国生理学会第24届全国会员代表大会暨生理学学术大会》
会议日期：2014年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
基 金：supported by the grant(№s 81200853,81171184,31060139,81100829 and 81260187)from National Natural Science Foundation of China the grant(№20111BBG70009-1)from Technology Pedestal and Society Development Project of Jiangxi Province
关 键 词：P2X3 receptor dorsal root ganglia neuropathic pain mlncRNA2Y
摘 要：Objective:Neuropathic pain is an intractable *** pathogenesis is associated with the abnormalities of gene transcription and translation in the sensory *** RNA like non-coding RNAs（mlnc RNAs）as a new member of gene regulation is being more and more attention,but how mlnc RNAs regulate the neuropathic pain is unclear.P2X3 receptor is closely related to the development of neuropathic *** study will be to explore the effect and possible mechanisms of m RNA-like non-coding RNA2Y（mlnc RNA2Y）on P2X3 receptor-mediated neuropathic pain by sciatic chronic constriction injury（CCI）rats ***:The changes of the paw thermal latency（TWL）and the mechanical withdrawal threshold（MWT）were *** expression levels of P2X3 receptor in dorsal root ganglion（DRG）were detected by Western Blot and Real-time quantitative *** expression levels of mlnc RNA2Y in DRG were detected by quantitative *** The behaviorresults of CCI rats showed that at 5 days after surgery,TWL and MWT on surgical side limbs of CCI operated rats were much lower than those in sham rats（p0.05）.Compared with the CCI rats,TWL and MWT in CCI treated with mlnc RNA2Y si RNA or A-317491 rats were increased continuously during 7-14days of post-operation（p0.01）,in contrast,TWL and MWT in control transfected with mlnc RNA2Y plasmid rats were *** PCR results showed that the expressions of mlnc RNA2Y and P2X3 in DRG of CCI rats and control transfected with mlnc RNA2Y plasmid rats were higher than those in sham rats,but the elevated expressions of mlnc RNA2Y and P2X3 in CCI rats treated with mlnc RNA2Y si RNA were decreased in comparison with those in CCI *** blot results showed that compared with sham rats,the expression levels of P2X3 protein in L4/L5 DRG of CCI and control transfected with mlnc RNA2Y plasmid rats were increased（p0.05）.However P2X3 expressions in CCI treated with mlnc RNA2Y si RNA or A-317491 rats were lower than those in CC