The bioinformaticsanalyses reveal novel antigen epitopesin Major Outer Membrane Protein(MOMP) of Chlamydia trachomatis
作者单位：State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890294) and Xinjiang Key Laboratory of Echinococcosis First Affiliated Hospital of Xinjiang Medical University College of Basic Medicine of Xinjiang Medical University Departments of Microbiology and Immunologya and Pathology University of Texas Health Science Center at San Antonio
会议名称：《第十届全国免疫学学术大会》
会议日期：2015年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：C.trachomatis MOMP prokaryotic expression antigen epitope bioinformatics
摘 要：Purpose: The aim of this study was to predict the T-cell and B-cell epitopes in Major Outer Membrane Protein(MOMP) of Chlamydia trachomatis(C. trachomatis) by using online software, and also analysis of the secondary structure of MOMP through bioinformatics ***: The predictions of secondary structure of MOMP protein were carried out by using SOPMA software,andthe prediction of B-cell epitopes in MOMP protein were using IEDB and LEPS software, while the T-cells epitopes were predicted by the software of IEBD and SYFPEITHI. The predictions from the software were combined with MOMP protein characteristics,including surface features, hydrophilic, flexibility, accessibilityand plasticity,to analyze the common epitopes areas response by T-cells and ***:In the secondary structure of C. trachomat is MOMP,the alpha-helices were accounted for 41.62% of total amino acid, while the beta sheets and random coil were accounted for 19.80% and 32.49%, respectively. Predictions combined with MOMP protein surface features, hydrophilic, flexibility, accessibility and plasticity were further characterized,and three highscore B-cell epitope areas were found as located in24