Effect of electroacupuncture at Zuansanli(ST36) on dorsal randompattern skin flap survival in a rat model
作者单位：温州医科大学附属第二医院
会议名称：《2014年浙江省医学会手外科暨显微外科学学术年会》
会议日期：2014年
学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学]
关 键 词：flap survival skin necrosis Zuansanli(ST36) random-pattern skin flap electroacupuncture
摘 要：Introduction Acupuncture especially is one of the most frequently requested of the complementary therapies in western countries *** at Zusanli(ST36)could accelerate angiogenesis by up-regulating HIF-1 protein,and enhance skin microcirculation blood *** addition,it was used to antioxidant,which can inhibit the formation of *** paper aims to study how electroacupuncture at Zuansanli(ST36)effects on randompattern flap survival rate through animal *** and methods:Thirty female Sprague-Dawley rats were randomly divided into three groups:Control(no postoperative electroacupuncture)and Study A(postoperative electroacupuncture at non-acupoint areas,10 Hz for 30 min per day)and Study B(postoperative electroacupuncture at Zusanli,10 Hz for 30 min per day).TheMcFarlane flaprat models were established on the rat *** 7days’electroacupuncture,the flap survival area ratio was *** tissue samples were taken for histological *** VEGF,MDA and SOD expression were detected by ***:7 days after operation,the flap necrotic areas ratio in control group(66.65±2.81)%and in study A(64.65±2.42)%were significantly enlarged compared with that of the study B(48.81±2.33)%(P0.01).Histological analysis demonstrated angiogenesis with mean vessel density per mm2 being lower in control group(15.4±4.4)and in study A(17.4±4.3)than in control group(27.2±4.1)(P0.05).VEGF expression and SOD contents were significantly increased in study B Group compared with study A Group and control group(p0.01),whereas MDA level was reduced(p0.05).Conclusions:Electroacupuncture at Zuansanli(ST36)may increase the expression of VEGF,which can effectively increase angiogenesis to provide inadequate vascular *** block the release of ischemia-reperfusion injuryassociated free radical,as well as the production of the superoxide *** addition,the up-regulated SOD and the down-regulated MDA are evidence