Changes of genes activating cold exposure-induced thermogenesis in brown adipose tissue of mice by RNA-seq

作     者：Xiaonuan Li~2 Yingfeng Luo~1 Linlin Pan~2 Tongwu Zhang~2 Songnian Hu~(1 2) and Jun Yu~(1 2) 1 CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing China 2 James D.Watson Institute of Genome Sciences Zhejiang University Hangzhou China 

会议名称：《2011国际基因组学大会》

会议日期：2011年

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

关 键 词：Brown adipose tissue(BAT) cold next-generation sequencing 

摘      要：正Brown adipose tissue（BAT） is a specialized tissue that can dissipate energy through the uncoupling effect of UCP1 and therefore is a therapeutic target to counteract obesity-associated *** identify genes activating the nonshivering thermogenesis of BAT,we performed RNA-Seq of mRNA samples collected from the BAT of cold-exposed mice（24h）（Bat-cold） and of mice kept at room temperature （Bat-normal） using next-generation sequencing technology（SOLID,Life Technologies）.We generated more than 2xl07 uniq-mapped tags for each sample;these tags were annotated to 15471 Bat-cold genes and 15202 Bat-normal genes based on mouse Ensembl *** identified 846 differentially expressed genes（q-value0.001,FC2,and absolute change50） by DEGseq program and found that 588 genes were down-regulated and 258 were up-regulated during cold *** up-regulated genes were over-represented in the Cholesterol Biosynthesis pathway and the Fatty Acid Biosynthesis pathway and the down-regulated genes were over-represented in the Apoptosis *** also considered genes expressed in intergenic regions of the mouse genome and identified about 5000 fragments in each *** results provide molecular evidence for the theory that during acute cold exposure the number of brown adipocytes increases through promoting proliferation and preventing apoptosis.