Identification of major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Xiang-Fu-Si-Wu Decoction fraction by UPLC-Q-TOF-MS and MetabolynxTM
会议名称：《中华中医药学会中药分析分会第五届学术交流会》
会议日期：2012年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Xiang-Fu-Si-Wu Decoction Metabolites UPLC-Q-TOF-MS MetaboLynx
摘 要：A simple and specific UPLC-Q-TOF-MS method with automated data analysis(MetaboLynx) has been developed and applied for the primary investigation of metabolism pathway of active Xiang-Fu-Si-Wu Decoction fraction(XFSW-8).Through the online ESI-MSE analysis and automated data analysis(MetaboLynx), a total of fifteen constituents have been identified or tentatively characterized from the nine tested samples after oral administration of *** parent compounds and nine metabolites were identified in blood and eight *** / demethylates sulfation,glucuronidation,sulfation and glucuronidation conjugates were the major metabolic pathways of alkaloids in XFSW-8 in *** present study provided important information about the metabolism of alkaloids in Xiang-Fu-Si-Wu Decoction which will be helpful for fully understanding the mechanism of action of this Chinese medicine.