Subdomain-Specific Collapse of Denatured Staphylococcal Nuclease Revealed by Single Molecule Fluorescence Resonance Energy Transfer Measurements
作者单位：National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences15 Datun Road
会议名称：《第六届全国化学生物学学术会议》
会议日期：2009年
学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学]
基 金：supported by the grants 20673002,20733001,and 30620130109 the Chinese Natural Science Foundation and 2006CB910903,2006CB910304,and 2006CB806508 the Chinese Ministry of Science and Technology
摘 要：正By using single molecule fluorescence resonance energy transfer（smFRET）,the equilibrium denaturation of staphylococcal nuclease（SNase） induced by guanidinium hydrochloride（GdmCl） has been *** have characterized the collapse of the denatured chain and its relation to structure *** mutants,K28C/H124C and K28C/K97C,were constructed and labeled for