Long non-coding RNA Profiling in Laryngeal Squamous Cell Carcinoma and its Clinical Significance:Potential Biomarkers for LSCC
会议名称：《2014年浙江省医学会耳鼻咽喉头颈外科学学术年会》
会议日期：2014年
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
关 键 词：laryngeal cancer lncRNA expression profile AC026166.2-001 RP11-169D4.1-001
摘 要：Long non-coding RNAs(lncRNAs)are novel transcripts that may play important roles in *** study aimed to resolve the lncRNA profile of larynx squamous cell carcinoma(LSCC)and to determine its clinical *** global lncRNA expression profile in LSCC tissues was measured by lncRNA *** expressed lncRNAs were identified and levels of AC026166.2-001 and RP11-169D4.1-001lncRNAs in 87 LSCC samples and paired adjacent normal tissue were analyzed by real-time quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR).The clinical significance of these lncRNAs in laryngeal cancer was analyzed and survival data were estimated by the Kaplan-Meier method and the logrank test.A receiver operating characteristic(ROC)curve was constructed to check the diagnostic *** lncRNA expression profile of tumor samples,684 lncRNAs were upregulated and 747 lncRNAs were downregulated(fold-change2.0).Of these,AC026166.2-001 and RP11-169D4.1-001 were distinctly dysregulated.,with AC026166.2-001 exhibiting lower expression in cancer tissues and RP11-169D4.1-001higher *** verified that both AC026166.2-001 and RP11-169D4.1-001 were expressed at a lower level in cervical lymph nodes compared with paired laryngeal cancer tissues and paired normal ***11-169D4.1-001 levels were positively correlated with lymph node metastasis(P=0.007).From the survival analysis,decreased levels of AC026166.2-001 and RP11-169D4.1-001 were associated with poorer *** area under the ROC curve was up to 0.65 and 0.67,respectively,and the cut-off point of ACt was 11.23 and 10.53,***026166.2-001 and RP11-169D4.1-001 may act as novel biomarkers in LSCC and may be potential therapeutic targets for LSCC *** AC026166.2-001 and RP11-169D4.1-001 could be independent prognostic factors for survival in LSCC.