Microbial diversity and community structure of natural Ophiocordyceps sinensis and its microhabitats
作者单位：Key Laboratory of Urban Agriculture(South) Ministry of AgriculturePlant Biotechnology Research CenterSchool of Agriculture and BiologyShanghai Jiaotong University Department of Ecology and Evolutionary BiologySchool of Life SciencesFudan University School of Public HealthGuangdong Medical College
会议名称：《中国菌物学会2015年学术年会》
会议日期：2015年
学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学]
关 键 词：Ophiocordyceps sinensis microbial community bioresource diversity
摘 要：Background:Ophiocordyceps sinensis（DongC hong Xia Cao（DCXC） in Chinese）, a fungal parasite of caterpillars, is a traditional Chinese medicine. Bioactive components isolated from DCXC possess a wide range of pharmacological actions. Many efforts have been directed towards isolating the fungi based on culture-dependent methods for investigation of fungal diversity in order to confirm the anamorph of DCXC and find new medicinal fungi resources, and the results have been varied. Results: A total of 44,588 bacterial and 51,584 fungal sequences corresponding to 11694 and 9297 putative operational taxonomic units（OTU） were respectively identified by a Roche/454-based, high throughput sequence analysis of 16 S r RNA genes and ITS regions. The main bacterial groups were Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria and Firmicutes, while the Ascomycota, Basidiomycota and Zygomycota were the main fungal phyla. Proteobacteria presented 68.4%, 49.5%, 38.9% and 35.6% of all bacteria in the sclerotia, stromata, external mycelial cortices and soil, respectively. As the main fungi phyla, Ascomycota presented 21.0%, 45.6%, 26.4% and 59.3% in the sclerotia, stromata, external mycelial cortices and soil, respectively. Bacterial and fungal communities were more diverse in the environmental sample than in the DCXC sample. The microbial communities were obviously distinct in each sample. Several novel unclassifiable bacterial（10.41%） and fungal（37.92%） species were also detected. Conclusions: This study identified numerous microorganisms associated with DCXC and its microhabitats, some of fungi have been previously identified while bacteria represent new candidates. Our analyses suggested that the diversity of bacteria and fungi makes DCXC an integrated microecosystem. Thus, DCXC represents multiple microorganisms, not a single fungus.