Three-dimensional collagen scaffold culture promotes the differentiation of bone marrow cells into regulatory dendritic cells
作者单位：State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Public Health of Agricultural Ministry Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Reproductive and Genetic Center of National Research Institute for Family Planning
会议名称：《第十届全国免疫学学术大会》
会议日期：2015年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：bone marrow cells 3D culture collagen scaffold regulatory dendritic cells differentiation
摘 要：Objective:To study the effects of three-dimensional(3D)collagen scaffold on bone marrow cells to differentiate into ***:We cultured BMCs on the 3D collagen scaffolds and two-dimensional(2D)culture *** morphological characteristics of the differentiated DCs cultured on 2D and 3D collagen scaffold were imaged under optical microscope and Scanning Electron Microscopy(SEM).Next,we analyzed the expressions of CD11b,MHC-II and the co-stimulatory molecules CD40,CD80,CD86 and CD83 in i DCs and m DCs using flow cytometry on CD11c+*** then the phagocytosis ability of generated DCs,i DC-2D and i DC-3D were co-incubated with FITC–dextran and then the intensity of FITC taken up by the DCs was measured using flow cytometry on CD11c+*** addition,the T-cell proliferation was assessed using flow ***,the cytokines profiles of generated DCs were collected to measure IL-10,IL-12p70,and TNF-αby ELISA ***,we evaluated the suppressive effects of the generated DCs in vivo delay type hypersensitivity(DTH)in C57BL/6 ***:Our results showed that differentiated DCs derived from 3D collagen scaffold presented with high expression of CD11b and low expressions of CD11c,co-stimulator and MHC-II molecules compared with 2D ***,these cells possessed a weak antigen uptake ability and suppression of T-cell proliferation in ***,they displayed a potent immunoregulatory function to alleviate allo-delay type hypersensitivity(allo-DTH)in ***:The 3D culture provides a suitable niche in specialized DCs *** researches demonstrated that 3D collagen scaffold culture promotes the differentiation of BMCs into regulatory DCs,CD11bhi MHClo *** differentiated CD11bhi MHClo DCs possessed the capacity to negatively regulate immune response in vitro and in vivo,whereas a high level of IL-10 production.