Expression of the neural specific protein,GAP-43,dramatically lengthens the cell cycle in fibroblasts

作     者：Ya-Juan YAO,Jun-Peng ZHAO,Qun-Yuan XU~* Beijing Institute for Neuroscience,Capital medical University,Beijing Center of Neural Regeneration & Repair,Key Laboratory for Neurodegenerative Disease of the Ministry of Education,Beijing 100069 

会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》

会议日期：2009年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

关 键 词：neurogenesis cell cycle GAP-43 BrdU NIH 3T3 cells 

摘      要：正Objective It has been demonstrated that during neurogenesis in the mammalian brain,cell cycle lengthening in neuronal progenitors may cause them to switch from proliferation to neuron-generating ***,little is known about the cellular mechanisms involved in lengthening of the cell ***-associated protein-43（GAP-43） is a nervous system-specific protein whose expression in proliferating neuroblasts is related to *** this study, we investigated the effect of GAP-43 on cell cycle progression in transgenic fibroblast *** By using cumulative bromodeoxyuridine labeling,the cell-cycle kinetics in GAP-43-transgenic and control NIH 3T3 cells were analyzed. Results Our data demonstrated that the expression of GAP-43 in fibroblasts resulted in lengthening of the cell cycle compared to control *** The mechanism by which GAP-43 mediated this effect appeared to be involved in lengthening of the cell cycle.