Rapid Detection of wheat yellow mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification

作     者：Zhang Zong-ying,Liu Xiao-jun,Li Da-wei,Yu Jia-lin,Han Cheng-gui (Department of Plant Pathology and State Key Laboratory for Agrobiotechnology, China Agricultural University,Beijing 100193,P.R.China) 

会议名称：《中国植物病理学会2011年学术年会》

会议日期：2011年

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported partially by National Department Public Benefit Research Funds(nyhyzx07-051 and 2008ZX08002-001) 

关 键 词：wheat yellow mosaic virus reverse transcription 

摘      要：正For the detection of Wheat yellow mosaic virus（WYMV）,a reverse transcription loop-mediated isothermal amplification（RT-LAMP） method was *** Primer Explorer software,four sets of primers were designed to carry out RT-LAMP for WYMV *** sets of primers were *** set IV primers were chosen randomly for developing the specificity and sensitivity of RT-LAMP for WYMV,and detecting WYMV for the field *** specificity test indicated that the WYMV primers could amplify WYMV but not Chinese wheat mosaic virus and barley stripe mosaic virus ***