Study on CCK-8 participated modulation of algesia in caudate nucleus and mechanism of morphine dependence

作     者：Chun-Xiao YANG~1,Qing-Cheng LIANG~1,He-Ren GAO~2,Duo ZHANG~2,Guang-Wen ZHANG~2,Yu LIANG~2,Ying HANG~2, Man- Ying XU~(2,*) 1 Department of Neurology of 2nd Affiliated Hospital 2 Laboratory of Neural Electrophysiology,Department of Physiology,Harbin Medical University,Harbin 150081, China 

会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》

会议日期：2009年

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

关 键 词：cholecystokinin octapeptide caudate nucleus pain related neuron withdrawal symptom morphine depen- dence 

摘      要：正Objective To investigate the effect of caudate nucleus（Cd） on pain transmission and morphine dependence and the influence of cholecystokinin octapeptide（CCK-8） on pain,morphine dependence and withdrawal symptoms. Methods The electrophysiology,morphology and praxiology were used in the ***（1） In normal rats, CCK-8 resulted in increase in the net-increased value（NIV） of evoked discharge frequency,decrease in the average latency of the pain-excitatory neuron（PEN）,and prolonged inhibitory duration,reduced the NIV of the pain-inhibitory neuron（PIN）.L-365,260,antagonist of CCK-B receptor,antagonized the enhanced effects of PEN,the reduced effects of ***-8 played an excitatory role in the Cd pain transmission in normal rats.（2） Six days after administration of morphine continuously,spontaneous withdrawal symptoms increased obviously during withdrawal 24～72 h in morphinistic *** withdrawal 40h,spontaneous withdrawal symptoms increased much more obviously in morphinistic rats than in normal ones（P0.01）.（3） On withdrawal 24h of morphinistic rats,CCK-8 or L-365,260 was injected into the Cd after observed spontaneous withdrawal *** withdrawal 40h,Total score of withdrawal symptoms obviously increased in morphine+CCK-8 rats,and significantly decreased in morphine+L-365,260 rats respectively compared with morphinistic rats group（P0.01）.（4） In morphinistic rats,CCK-8 resulted in the increase in the NIV of evoked discharge frequency,the decrease in the average latency of PEN,and prolonged inhibitory duration,reduced the NTV of PIN（P0.05, P0.01）.L-365,260 antagonized the enhanced effects of PEN,the reduced effects of ***-8 played an excitatory role in the Cd pain transmission in morphinistic *** Exogenous CCK-8 could potentialize the responses of the pain-related neurons in Cd of normal and morphinism rats to the noxious stimulation,exhibiting the facilitated effect on *** effects of CCK-8 were mainly mediated by CCK-B recept