FGF13A represses transcription of rRNA genes as a nucleolar protein
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
关 键 词：iFGF UBF1 ribosomal RNA transcription neurite outgrowth
摘 要：Objective Fibroblast growth factors(FGFs) are among the first-indentified families of growth *** are polypeptides with diverse biological activities including cell proliferation,differentiation,migration and so *** their functions are much more evident in the nervous system than in *** FGFs have N-terminal signal peptides and can be secreted ***,FGF11 subfamily(FGFs 11-14) is a special *** FGFs lack signal peptides, remain intracellularly and function within cells in a receptor-independent manner,hereafter referred to as *** growth factor 13(FGF13) is a member of the intracellular FGF homologous factor subfamily(iFGFs) and highly expressed in nervous system,however its functions are still *** The subcellular localization of FGF13A was investigated by *** interaction between FGF13A and UBF1 was investigated by coimmunoprecipitation (co-IP) *** function of FGF13A on the neurite outgrowth of DRG neurons was investigated by electroporation and ***(1) The expression of FGF13A-EGFP in transfected ND7-23 cells or dissociated primary DRG neurons was highly enriched in the nucleolus of the two types of cells and colocalized with the nucleolus marker,fibrillarin and nucleolin.(2) Overexpression of FGF13A repressed rRNA transcription through interacting with upstream binding factor 1(UBF1),a RNA pol I transcription factor.(3) Overexpression of FGF13A significantly inhibited neurite outgrowm of dissociated DRG *** Nucleolus-localized FGF13A represses rRNA transcription and therefore inhibits DRG neurite outgrowth.