Development of multiplex real-time PCR for simultaneous detection of three Potyviruses in tobacco plants
会议名称：《中国植物病理学会2012年学术年会》
会议日期：2012年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
基 金：supported by the Key Technology Program of China National Tobacco Corporation(110200902046) the 111 Project from the Education Ministry of China,(No.B07049 )
关 键 词：Tobacco etch virus Potato virus Y Tobacco vein banding mosaic virus Multiplex real-time PCR
摘 要：正To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus（TEV）,Potato virus Y（PVY） and Tobacco vein banding mosaic virus（TVBMV）.Specific primer and probe combinations for TEV and TVBMV were developed from the coat protein region of the viral *** detect PVY,a primer and probe combination PVY-Univ F,PVY-Univ R and PVY-Univ P for amplifying the coat protein region of the virus genome was *** detection limit of multiplex real-time PCR for these viruses was 10 copies number/μl of the standard *** multiplex reaction was successful in detection of these three pathogens,with no non-specific amplification and cross-reaction was *** multiplex real-time PCR provides a rapid, effective,specific and sensitive method for the simultaneous detection and quantification of the three pathogens on infected tobacco *** multiplex real-time PCR not only will be useful for diagnostic, ecological,epidemiology and pathogenesis studies,and investigations of host/virus or virus/virus interactions, especially when the host is infected with more than one virus.