Ceruloplasmin is involved in the nigral iron accumulation of 6-OHDA lesioned rats
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
关 键 词：6-hydroxydopamine Ceruloplasmin iron Parkinson’s disease
摘 要：正Objective Elevated iron levels in the substantia nigra participate in neuronal death in Parkinson’s disease（PD）. While the mechanisms underlying the increased iron are still ***（CP） is a ferroxidase that converts highly toxic ferrous iron to its non-toxic ferric form,which cooperated with ferroportinl（FP1） facilitating the export of iron from reticuloendothelial system and other peripheral *** previous study showed that decreased expression of FP1 in the substantia nigra can account for the increased iron *** elucidate if the abnormal expression of CP is also involved in the nigral iron accumulation,here,we investigated CP expression in the substantia nigra of rats lesioned by 6- hydroxydopamine（6-OHDA）.Methods Immunofluorescence,immunohistochemistry and RT-PCR were used to observe the expression of ***（1） Using immunofluorescence,we showed mat FP1 and CP colocalized in the rat substantia nigra.（2） By immunohistochemistry,we observed that one day after 6-OHDA lesion,the expression of CP decreased compared with the *** rats began showing rotation behavior induced by apomorphine,usually after six weeks since 6-OHDA lesion,they are considered PD *** these PD models,a further decrease was observed.（3） RT-PCR showed that the mRNA level of CP decreased one day after 6-OHDA lesion compared with the control,and further decrease was also observed in the PD model *** These results show that FP1 and CP colocalize in the rat brain,indicating the coordinated actions of the two proteins in the iron export from neural cells;and suggest that decreased expression of CP in the substantia nigra is involved in the nigral iron accumulation of 6-OHDA lesioned rats.