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Use of a standardized cell culture assay to assess activitie...

Use of a standardized cell culture assay to assess activities of Composite Artemisa Capillaris Tablet against hepatitis B virus replication in vitro

作     者:Shan Li-mei,Zhao Yan-ling,Xiao Xiao-he (PLA Institute of Chinese Materia Medica,302 Hospital of PLA,Beijing,100039) 

会议名称:《中华中医药学会中成药学术研讨会》

会议日期:2007年

学科分类:1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

关 键 词:Composite Artemisa Capillaris Tablet 2.2.15 cells surface antigen e antigen HBVDNA 

摘      要:Objective:To explore the activities of Composite Artemisa Capillaris Tablet(复方茵陈片)against hepatitis B virus ***:By means of radioimmunoassay,Dot blot and Southern blot,the surface and e antigen production of 2.2.15,HBV DNA in 2.2.15 cell culture medium and in 2.2.15 cells were examined ***:HBsAg,HBeAg values of 2.2.15 cells treated by CACT were lower than that of control,the HBV DNA quantities in culture medium and in 2.2.15 cells decreased compared with the cells without treatment of ***:The results indicated that CACT could inhibit HBV replication, showing its potential antiviral activity in hepatitis B treatment.

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