Aberrant expression of the IFN regulatory factor 4-binding protein (IBP) in human colorectal cancer

作     者：Chuanmin Hu Zhujun Zhang Peng Li Qingliang Wang Yu Zhou Tao Fu Shuhui Li Weijing Yi Xinjun Li Yang Zhang Mingzhen Yang Xiuwu Bian Ji Ming Wang Guoguang Ying An Chen (a Department of Clinical Biochemistry Laboratory Science,Third Military Medical University,Chongqing 400038,PR China b Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038,PR China c Laboratory of Molecular Immunoregulation,Cancer and Inflammation Program,Center for Cancer Research,National Cancer Institute at Frederick,Building 560,Room 31-76,Frederick,MD 21702-1201,USA d Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,PR.China) 

会议名称：《第九届西南三省一市生物化学与分子生物学学术交流会》

会议日期：2008年

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Natural Science Foundation of China (No.30471577 and No.30570373) Chongqing Science & Technology Commission,Chongqing,PR,China (No.2007BB5068 and 2007BB5070) and Clinical innovation foundation 

关 键 词：IFN regulatory factor 4-binding protein (IBP) Monoclonal antibody Polyclonal antibodies Colorectal cancer 

摘      要：Recent studies shown that the IFN regulatory factor 4-binding protein (IBP) plays multiple biological roles in the activation,proliferation and differentiation of immun *** order to explore the potential role of IBP in human diseases,The murine hybridoma 2A2 specific for human IBP was obtained epitope *** found that IBP is not only expressed in immun cells as relevant to its functional studies so far executed,but also in human colon cancer cell lines by immunoblotting and *** strain mAb does not react with native IBP in immunocytochemistry (ICH) or immunohistochemistry (IHC).To further evaluate the significance of IBP expression in human colorectal cancer,we generated polyclonal antibodies with high specificity and sensitivity against human IBP through affinity *** systematic assay revealed that 42 cases were IBP-positive in 63 colorectal cancer tissues,while IBP expression was not detectable in control normal tissues with the same origin (p0.001).Moreover,IBP expression is related to the differentiation of colorectal cancer cells (p0.05).Taken together,with the Abs generated in this report,future works related to IBP functional characterization,and its potential role in colorectal cancer development would be greatly facilitated.