Chimeric DNA vaccines encoding Eimeria acervulina macrophage migration inhibitory factor(***)induce partial protection against experimental Eimeria infection
作者单位：南京农业大学动物医学院
会议名称：《中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会》
会议日期：2015年
学科分类：090602[农学-预防兽医学] 09[农学] 0906[农学-兽医学]
基 金：国家自然科学基金项目(31372428,31201896) 江苏高校优势学科建设工程资助项目
关 键 词：E.acervulina orthologue of macrophage migration inhibitory factor DNA vaccine genetic adjuvants recombinant protein
摘 要：Chimeric DNA vaccines co-expressing Eimeria acervulina macrophage migration inhibitory factor(***) and chicken IL-2(IL-2) or interferon-γ(IFN-γ) were constructed and their efficacies against *** were *** open reading frame(ORF) of *** was cloned from *** merozoites and subcloned into the eukaryotic expression vector pVAX1 with chicken cytokine gene IFN-γ or IL-2 to construct the DNA vaccines ***-IFN-γ,***-IL-2 and *** in vivo transfection of the target genes was detected by use of reverse transcription polymerase chain reaction(RT-PCR) and Western *** were carried out by vaccinating chickens twice with a dose rate of 100 μg *** days post second immunization,all chickens except the unchallenged control group were challenged orally with l×10