SHP-2 promotes the maturation of oligodendrocyte precursor cells through Akt and ERKl/2 signaling in vitro
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：SHP-2 oligodendrocyte precursor cells maturation Akt ERK1/2
摘 要：正Objective Oligodendrocyte precursor cells（OPCs）differentiate into oligodendrocytes（OLs）,which are responsible for ***,the molecular mechanisms of maturation and myelination by oligodendrocytes remain *** present study is aim to explore the roles of SHP-2 in oligodendrocytes *** In this study,we first searched the potential tyrosine phosphatases involved in oligodendrocytes maturation using OPCs as the ***,we characterized SHP-2 expression during the development of oligodendrocytes by RTPCR, immunostaining and Western *** roles of SHP-2 in olidendrocytes maturation were detected by gene manipulations ***,the mechanism was examined by detecting activities of concerned kinases. Results（1）We found that SHP-2 was persistently expressed during developmental process of oligodendrocytes.（2） SHP-2 knock down attenuated oligodendrocytes maturation and this effect was triiodo-L-thyronine（T3）dependent. Over-expression of SHP-2 promoted maturation in primary cultured oligodendrocytes.（3）It has been identified that SHP-2 may regulate oligodendrocytes maturation through Akt and Erkl/2 *** SHP-2 promotes oligodendrocytes maturation via Akt and ERK1/2 signaling in vitro.