Application of scorpion venom peptides in tumor and virus research
作者单位：State Key Laboratory of Virology College of Life Sciences Wuhan University Department of Neurosurgery University Hospital Erlangen Friedrich-Alexander University (FAU)of Erlangen-Nürnberg
会议名称：《第12届生物毒素研究及医药应用学术大会》
会议日期：2015年
学科分类：1007[医学-药学(可授医学、理学学位)] 100701[医学-药物化学] 10[医学]
基 金：supported by grants from National Science Fund for Excellent Young Scholars(Nos.31422049 and 31572289) Hubei Science Fund for Excellent Scholars(No. 2015CFA042) Fundamental Research Fund for the Central Universities in China(Nos. 2042015kf0255 and 2042014kf0205) China Scholarship Council(No.201308420306)
关 键 词：Scorpion venom peptide Serine protease inhibitor Glioblastoma Antiviral peptide HBV HCV Endosomal escape Signal pathway
摘 要：A new peptide precursor,termed Sj7170,was characterized from the venomous gland cDNA library of the scorpion ***7170 was deduced to be a 62 amino acid peptide cross-linked by five disulfide *** recombinant Sj7170 peptide（rSj7170）with a chromatographic purity was produced by prokaryotic expression *** inhibition assay in vitro and in vivoshowed that rSj7170 specifically inhibited the activity of α-chymotrypsin at micromole *** addition,Sj7170 not only promoted cell proliferation and colony formation by up-regulating the expression of cyclinD1 in vitro,but also enhanced tumor growth in nude ***,Sj7170 accelerated cellular migration and invasion by increasing the expression of the transcription factor Snail and then inducing the epithelial-mesenchymal ***,Sj7170 changed cell morphology and cytoskeleton of U87 cells by GTPase *** together,Sj7170 is a unique dual-functional peptide:a specific α-chymotrypsin inhibitor and a potent tumorigenesis/*** work not only opens an avenue of developing new modulators of tumorigenesis/metastasis from serine protease inhibitors,but also strengthens the functional link between protease inhibitors and tumor *** brain tumors are hallmarked by fast proliferation and their ability to destruct the brain microenvironment for their growth *** chemotherapeutic strategies are currently lacking which combat this deadly disease *** glioma-specific chloride ion channel（GCC）represents a specific target for therapy and Chlorotoxin（CTX）,a peptide derived from scorpion venom has been shown to be efficacious and specific in blocking glioma CI- channel ***,we report on one new derivative（termed CA4） based on the CTX peptide sequence with high potency against glioma cell ***4 is effective in reducing glioma cell proliferation and cell ***,we found that CA4 and CTX are selective and s