Dextran prevents TE-induced intracellular Ca2+overload in isolated adult mouse ventricular myocytes
作者单位：Department of Marine BiotechnologyFaculty of Naval MedicineSecond Military Medical University Grade 2013Naval Clinical MedicineSecond Military Medical University Grade 2013Clinical MedicineSecond Military Medical University
会议名称：《第12届生物毒素研究及医药应用学术大会》
会议日期：2015年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：General Projects of the National Natural Science Foundation of China(81470518) Youth Science Project of National Natural Science Foundation of China(81401578)
关 键 词：Jellyfish Cyaneacapillata tentacle extract Ca2+ Dextran
摘 要：Intracellular Ca overload plays a pivotal role in acute heart failure,which is induced by tentacle extract(TE) from jellyfish *** an intervention that inhibits the increase of cytosolic Cafrom both extracellular Ca entry and intracellular Ca release,it is maybe beneficial to the antagonistic effect of the cardiotoxicity of jellyfish ***,the polysaccharide,Dextran,with its large molecule weight 0 000 kDa and capacity to stabilize the cell function,might be able to prevent the TE-induced intracellular Ca overload by hindering the extracellular Ca entry,through the previously reported non-selective ion channel complex assembled by jellyfish venom in the cell *** mouse ventricular myocytes were isolated by the method collagenase II *** were loaded with Fluo-4 to indicate the Ca in *** Ca imaging was scanned by an Olympus confocal microscopy with the excitation wavelength 488 nm and emission wavelength longer than 505 *** myocytes were gradually turning shrunk,accompanied with intracellular Caincrease after TE administration within 10 *** cells were pretreated with 5 μg/ml Dextran,intracellular Ca and morphological characteristics of cells did not change within 10 min after perfusion with 30 μg/ml ***,no obvious difference was observed in control andDextran *** conclusion,5 μg/ml Dextran prevents TE-induced intracellular Ca overload in isolated mouse ventricular myocytes,which is a promising reagent to antagonize the cardiotoxicity of jellyfish venom.