Discrimination of Alicyclobacillus Strains Using Nitrocellulose Membrane Filter and Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
作者单位：College of Food Science and EngineeringNorthwest A&F University School of Food ScienceWashington State UniversityPullmanWAUSA
会议名称：《第五届中国畜牧科技论坛》
会议日期：2011年
学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
基 金：supported by China State“11 th Five-Year Plan”Scientific and Technological Support Scheme(2006 BAK02A18,2006BAK02A24, 2006BAK02A05) Beyond Plan of the Ministry of Agriculture,China(2005-4) New Century Talent Plan of the Ministry of Education,China(2005) Special Major Science and Technology Project in Shaanxi Province,China(2006 KZ09-G1) China Scholarship Council the Agricultural Research Center,Washington State University
关 键 词：Alicyclobacillus spp. Microbial discrimination Fourier transform infrared spectroscopy nitrocellulose membrane filter linear discriminant analysis
摘 要：Alicyclobacillus *** thermoacidophilic,spore-forming bacteria,some of which cause spoilage in pasteurized and heat-treated apple juice products through the production of *** would be helpful if a rapid method to detect and discriminate Alicyclobacillus strains was available.A simple and rapid sample preparation method using nitrocellulose membrane filter(NMF) and a single reflection horizontal attenuated total reflection(HATR) accessory with FT-IR was developed *** transform infrared(FT-IR) spectroscopy was used and tested on eight Alicyclobacillus strains(KF,WAC,NWN-13501,NWN-12697,NWN- 12654,NWN-10682,1016,1101).A linear discriminant analysis(LDA) was established to discriminate Alicyclobacillus *** sample preparation method could successfully separated strains into different groups by principal component analysis(PCA).High identification accuracy(95%) was achieved with the LDA model.