Glutamate activated spinal astrocytes are involved in the thermal hyperalgesia
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
关 键 词：glutamate spinal astrocyte thermal hyperalgesia IL-6
摘 要：正Objective Numerous evidence suggests that spinal astrocytes play an important role in chronic pain through release of neuroactive substances,such as interleukin and *** produces marked effects in initiation and transmission of nociceptive ***,there is a lack of direct evidence that how glutamate activate astrocytes and what’s effects of activated astrocytes by glutamate on pain *** study therefore explored the direct effects of glutamate activation of spinal *** Primary culture of spinal astrocytes were administered with *** levels of protein and mRNA expression of IL-6,which reflect astrocyte activation, were determined by ELISA assay and real-time *** spinal astrocytes activated by glutamate were injected into the lumber enlargement of adult Wistar rats,and paw withdrawal latency（PWL） was *** Administration of glutamate（1 000 uM） activated astrocytes from 6 h after intrathecal injection and reached the maximal level at 24 h（the last time point we explored）.Besides,glutamate（1 000 uM） enhanced the release of IL-6 by astrocytes. Intrathecal injection of cultured spinal astrocytes,which were stimulated by glutamate,induced a substantial decrease of PWL,indicating the development of thermal *** Our results suggest that activated astrocytes by glutamate is sufficient to produce thermal hyperalgesia in rats by releasing IL-6.