ISOLATION OF SUBPROTOPLASTS VIA ULTRACENTRIFUGATION AND CREATION OF HYBRID CALLUS VIA CYTOPLAST-PROTOPLAST FUSION IN CITRUS

作     者：LIU Ji-Hong XU Xiao-Yong ZHANG Qing-Hua DENG Xin-Xin (National Key Laboratory of Crop Genetic Improvement National Center of Crop Molecular Breeding Huazhong Agricultural University Wuhan 430070 China) 

会议名称：《中国农业生物技术学会第三届会员代表大会暨学术交流会》

会议日期：2006年

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

摘      要：正Isolation of two kinds of subprotoplasts via ultracentrifugation in discontinuous sucrose gradient was conducted from Satsuma mandarin （Citrus unshiu Marc.）. Cytoplasts were successfully obtained when embryogenic protoplasts of Satsuma mandarin was centrifugated for 20 min at 38000 rpm at 25℃. DAPI staining did not detect nuclei that were present in normal protoplasts. The cytoplasts were 23.2 μm in size and their viability ranged from 75% to 85%. Microprotoplasts were isolated from partially synchronous