Cav1.2,Cav1.3,and Cav2.1 in the mouse hippocampus during and after pilocarpine-induced status epilepticus

作     者：Jie H.XU~1,Li LONG~3,Yong C.TANG~2,Hai T.HU~1,Feng R.TANG~(1,2,4) 1 Department of Anatomy and Histology,School of Medicine,Xi''an Jiaotong University,Xi''an 710061,China 2 Epilepsy Research Laboratory,National Neuroscience Institute,Singapore 3 Department of Cell Biology and Genetics,Kunming Medical College,Kunming 650031,China 4Department of Anatomy,National University of Singapore,Singapore 

会议名称：《中国神经科学学会第四次会员代表大会暨第七届全国学术会议》

会议日期：2007年

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

关 键 词：calcium channels intemeurons astrocytes hippocampus epilepsy 

摘      要：正Calcium binding proteins are well known to be expressed by different groups of hippocampal intemeurons;however,whether voltage-dependent calcium channels（Cav） are also localized in these neurons,changed during and after status epilepticus（SE）,and involved in epileptic activity have not been *** the present study,we showed the colocalization of three subtypes of voltage-gated calcium channels（Cav1.2,Cav1.3,or Cav2.1） with different calcium binding proteins such as calbindin（CB）,calretinin（CR）,and parvalbumin（PV）.At early stages during and after pilocarpine -induced status epilepticus（PISE）,significant changes of expression of Cav 1.2,Cav 1.3（L-type）,and Cav2.1（P/Q-type） were found in different groups of hippocampal *** expression of Cav 1.3 or Cav2.1 in reactive astrocytes was shown at 1 week and 2 months after *** the latter time point,higher percentages of colocalization of PV and Cav1.2,CB,or PV and Cav1.3 or Cav2.1,lower percentages of CR and Cav 1.3 or Cav2.1 immunoposivie neurons were observed in gliotic CAl *** therefore conclude that voltage-gated calcium channels are expressed by different groups of hippocampal intemeurons in the *** acute stages during and after PISE,up- or down-regulation of Cav1.2,Cav1.3,or Cav2.1 in functionally different groups of intemeurons in CA1 area may be related to the changes of their ***-regulation of Cav 1.2,Cav 1.3,or Cav2.1 in granule cells may be directly related to the occurrence of SE. The induced expression of Cavl.3 or Cav2.1 in reactive astrocytes at 1 week and 2 months after PISE suggests that Cav1.3 or Cav2.1-related calcium signaling in reactive astrocytes may be involved in initiation,maintenance or spread of seizure *** gliotic CA1 area at chronic stage（i.e.,2 months after PISE）,the occurrence of higher percentages of colocalization of PV and Cav1.2,CB,or PV and Cav1.3 or Cav2.1,lower percentages of CR and Cav1.3 or Cav2.1 immunopositive neurons may suggest tha