Characterization of DREAM isoforms in astrocytes and neurons
作者单位：Neuroscience Research Institute Department of NeurobiologySchool of Basic Medical Sciences Key Laboratory for NeuroscienceMinistry of Education Key Laboratory for NeuroscienceMinistry of Public HealthPeking University
会议名称：《中国神经科学学会第九届全国学术会议暨第五次会员代表大会》
会议日期：2011年
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
基 金：supported by National Natural Science Foundation of China(30670644,30870818) 973 project (2011CB504400) Beijing Natural Science Foundation(7091004)
关 键 词：astrocyte neuron DREAM isoforms
摘 要：Objective Downstream regulatory element antagonist modulator(DREAM),as a multifunctional Ca binding protein,binds with specific DNA sequence to regulate gene expression and with Kv4.2 or presenilins outside the nucleus to regulate membrane excitability and calcium homeostasis,*** main isoforms of DREAM (A and B) were produced by alternative *** study is to observe the characterization of DREAM isoforms expression and distribution in astrocytes and *** Expression of DREAM A and B mRNA in primary culture of neural cells(astrocytes,GABAergic neuron and glutamatergic neuron) was measured by real time PCR. Subcloned DREAM isoform A or B in pIRES2-EGFP vectors and the plasmids were transfected into astrocytes to observe their subcellular *** We found that both DREAM A and B are present in all neural cells *** A was in a higher level in glutamatergic neuron whereas isoform B was in a higher level in astrocyte. Isoform A showed a diffusible distribution and isoform B appeared mainly localized in the *** mRNA levels of these two isoforms were measured in these neural cells at different *** found that isoform A showed the highest level in older but isoform B showed the highest level in younger *** confirmed these developmental changes in tissue of cerebral cortex and *** These data suggest that DREAM A is the major isoform in mature cells and can function as a transcriptional *** B is mainly localized in ER and may play a role in the early stage of development.