Visfatin upregulates the expression of monocyte chemotactic protein-1 via lectin-like oxidized low-density lipoprotein receptor-1 in vascular endothelial cells

作     者：Xiaoyu Han~(1,2),Wenchao Wu~1,Qian Wang~1,Xiaoying Chen~(1,2),Xiaojing Liu~(1,2) 1.Laboratory of Cardiovascular Diseases,West China Hospital,Sichuan University 2.Department of Cardiology,West China Hospital,Sichuan University,Chengdu 610041 China 

会议名称：《中国生理学会心血管生理学术研讨会》

会议日期：2011年

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

关 键 词：Visfatin lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) monocyte chemotactic protein-1(MCP-1) 

摘      要：正Background:Obesity is a major risk factor for the development of atherosclerosis,and atherosclerosis is recognized as a chronic inflammatory *** is identified as a novel visceral adipokine,which might be involved in obesity-related vascular diseases and ***-1,a receptor for ox-LDL（oxidized low-density lipoprotein）,has been implicated in ***,the potential link between Visfatin and LOX-1 has not been ***,whether visfatin could regulate LOX-1 expression in human umbilical vein endothelial cells（HUVECs） was investigated in this *** effect of blocking LOX-1 expression on the expression of monocyte chemotactic protein-1（MCP-1） was further ***:HUVECs were isolated from umbilical cords by collagenase digestion and cultured as previously ***-arrested HUVECs were treated with different concentrations of human recombinant Visfatin for different *** gene expression of LOX-1 and MCP-1 was analyzed by real-time RT-PCR,the protein expression of LOX-1 was assessed by Western Blot and immnuocytochemistry,and the secretion of MCP-1 was measured by ***:Compare with untreated control groups,100ng/mL concentration of Visfatin could induce LOX-1 and MCP-1 mRNA and protein expression after 24 hours treatment in *** up-regulation of MCP-1 expression appears to be LOX-1-dependent since blockade of LOX-1 by its specific inhibitor polyinosinic acid inhibits the visfatin-induced MCP-1 gene and protein expression Conclusions:Our findings suggest for the first time that, visfatin could increase the gene expression and protein production of MCP-1 via LOX-1 in HUVECs,which might play an important role in the pathogenesis of vascular inflammation and atherosclerosis.