Effect of continuous administration of corticosterone on sleep in rat
会议名称：《中国药理学会第十一次全国学术会议》
会议日期：2011年
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：The project supported by the grants from the National Scientific & Technological major special project(2009ZX09103-124) the National Natural Science Foundation of China(30640070 and 30772556) Research Fund from Ministry of Education of China(20100001110048) the 985 project from Peking University
关 键 词：HPA axis corticosterone sleep c-Fos
摘 要：OBJECTIVE To investigate the influence of persistently elevated corticosterone on sleep parameters in rat and to inspect the changes in sleep related nucleus in the brain,in order to elucidate how the dysfunction of HPA axis,always seen in mental disorders such as depression,affects *** Male SD rat was subcutaneously injected with 4 different dose of corticosterone for continuous 7 *** parameters were examined by EEG and EMG recording.C - Fos protein and specific neurotransmitter markers immunoreactivity in sleep- and wake-active nucleus were conducted with the method of *** Corticosterone administration at 10 mg·kg,20 mg·kg and 40 mg·kg for 7 d can significantly reduce the total sleep time and NREM sleep time without influence on SWS and REM *** dose of 0.5 mg·kg can only decrease light sleep time and NREM sleep time as a ***,the ratio of REM sleep time was remarkably increased after the treatment of 40 mg·kg corticosterone for consecutive 7 *** immunohistochemical experiments were processed at 40 mg·kg *** results show a decline of c-Fos expression in GABAergic neurons of VLPO(Ventrolateral preoptic area),and an increase of c-Fos immunoreactivity in noradrenergic neurons of LC(Locus coeruleus).CONCLUSION These findings demonstrate that the reduction of total sleep time and augmentation of REM sleep ratio,which resulted from incessant addition of corticosterone,were due to the alteration of sleep-wake related nucleus,for which decreased activity of GABAergic neurons in VLPO and increased activity of noradrenergic neurons in LC are most likely to responsible.