cPKCbetaⅡ-CRMP-2 pathway was involved in hypoxic preconditioning-induced neuroprotection against cerebral ischemic injury
会议名称：《中国神经科学学会第四次会员代表大会暨第八届全国学术会议》
会议日期：2009年
学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学]
基 金：National Natural Science Foundation of China(30670782 and 30871219) China 973 Program (2006CB504100) Beijing Natural Science Foundation(5072008) Key Scientific Developing Program of Beijing Municipal Commission of Education(KZ200810025012) Beijing Municipal Program for Hundred-Thousand-Ten Thousand Excellent Talents of New Century(Li J) Funding Project for Aca-demic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality(PHR200906116)
关 键 词：hypoxic preconditioning(HPC) middle cerebral artery occlusion(MCAO) classical protein kinase CbetaⅡ(cPKCbetaⅡ) proteomics neuroprotection
摘 要：正Objective To identify the classical protein kinase CbetaⅡ（cPKCbetaⅡ）- interacting proteins involved in hypoxic preconditioning（HPC） and explore its changes in middle cerebral artery occlusion（MCAO） induced brain injuries of *** Using our established HPC and MCAO mouse models,we applied the techniques of immunoprecipitation, two-dimensional electrophoresis and mass spectrometry to identify cPKCbetaⅡ-interacting ***-PAGE and Western blot were used to analyze the changes in protein phosphorylation and expression levels in HPC and MCAO. Results We found that collapsin response mediator protein-2（CRMP-2） could be coimmunoprecipitated with cPKCbetaⅡ,and the protein expression of CRMP-2 interacting with cPKCbetaⅡincreased significantly in the cortex of mice after *** phosphorylation level,not protein expression,of CRMP-2 decreased significantly after cerebral ischemia and partially be turned over by *** These results suggested that cPKCbetaⅡ-CRMP-2 pathway was involved in the neuroprotective effects of hypoxic preconditioning on cerebral ischemia.