Allochemical Inhibitory Effect of Gracilaria tenuistipitata （Rhodophyta） on the Photosynthetic Apparatus of *** as Probed by OJIP Chlorophyll a Fluorescence Measurements
会议名称：《2012年环境污染与大众健康学术会议》
会议日期：2012年
学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0804[工学-仪器科学与技术]
基 金：National Key Technology R&D Program (2012BAC07B05) National Natural Science Foundation of China (No. 31000240) the Fundamental Research Funds for the Central Universities of China(21611428)
关 键 词：Gracilaria tenuistipitata HABs Pyramimonas sp. Allelopathy Photosynthetic inhibition JIP-test
摘 要：The effects of the dried macroalga, Gracilaria tenuistipitata （Rhodophyta）, on photosynthesis of a highly stress-resistant microalga, ***., were studied. Pyramimonas sp. was cultured with dried G. lemaneiformis under controlled laboratory conditions. The chlorophyll a （Chl a） fluorescence transient O-J-I-P curve coupled with its specific parameters was established. The Chl a fluorescence transients were recorded in vivo at high time resolution and analyzed according to the JIP-test which can quantify photosystem II activity. A clear dose-dependent relationship was observed between the dried biomass of G. tenuistipitata and its inhibitory effect on Pyramimonas sp. A decrease in the O-J-I-P curve expressed as Chl a fluorescence intensity along with its specific parameters were observed, which was also time-dependent. The main photosynthetic inhibitory targets of the macroalga on the microalga, according to the JIP-test, can be expressed as a decrease in the number of active reaction centers and the blocking of the electron transport chain. The results of the present study suggest that dried fragments of G. tenuistipitata effectively inhibit photosynthesis in Pyramimonas sp. and could thus be potential candidates for use in the control and mitigation of harmful algal blooms.