Development of a LC-MS/MS method for the determination of norcantharidin in beagle plasma and its application to the pharmacokinetic study
作者单位：Department of Pharmaceutical SciencesZhejiang Chinese Medical University The First Affiliated HospitalZhejiang Chinese Medical University
会议名称：《2013全国中药与天然药物高峰论坛暨第十三届全国中药和天然药物学术研讨会》
会议日期：2013年
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
关 键 词：Norcantharidin Pharmacokinetics LC-MS/MS Beagle
摘 要：A sensitive,simple and selective LC-MS/MS method was developed and validated for the determination of norcantharidin(NCTD) in beagle *** extraction of NCTD was performed on the plasma in the presence of ribavirin as an internal standard(IS) using *** samples were separated on a C18 column(100×2.0 mm,3μm), gradient eluted with acetonitrile-water mobile phase containing 0.1%formic acid and