Balance between pro-and anti-inflammation cytokines of atherosclerosis induced by immunological and inflammatory stimulations in rats
会议名称：《第十五届国际药理学大会》
会议日期：2006年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
关 键 词：atherosclerosis immunology inflammation Antibody Array
摘 要：正 To observe the changes of rats’aorta induced by immunolgic and inflammatory stimulation and probe cytokine of the aorta with Cytokine Antibody Array. To induce foam cell formation in the aorta of rat by repeat intraperitoneal injections zymosan every 4 days for 8 weeks and ovalbuimn. 2. 5mg/kg BW every one week for 5 times after initial subcutaneous sensitization simultaneously. All rats were feeded with a cholesterol-rich diet including control. Transmission Electron Microscope. Cholesterol Test Kit. Turbidimetry of Polyethylene Glycol and RayBio Rat Cytokine Antibody Array Ⅰ were used to detect ultrastructural changes of aorta, serum total cholesterol（TC） levels, the changes of cytokines respectively. The ultrastructural changes were characterized by monocytes and smooth muscle cell migration with phagocytize lipid granule after 8 weeks. The TC levels were significantly higher than control （p 0.05）. Proinflammation cytokines for example IL - 6. etc. increased during the process of AS. In conclusion The balance between pro- and anti-inflammation cytokines play an important role in immunological and inflammatory mechanism of AS.