In vitro spermatogenesis from embryonic stem cells in a chemical defined serum-free and feeder-free culture system
会议名称：《2012全国发育生物学大会》
会议日期：2012年
学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学]
摘 要：Embryonic stem cells(ESCs),which derived from the inner cell mass in the blastocyst,show the totipotent characteristics to differentiate into all three germ layers,containing the gametes as sperms and *** of functional sperms from ESCs in vitro could offer an important technical platform for the research on fertilization and individual *** researches focused on generating sperms from ESC clones,embryonic bodies(EBs) or primordial germ cells(PGCs) by endogenous control of gene expression or exogenous control of defined ***, low efficiency of the introduction process from ESCs or EBs and unrepeatable performance have hampered progress with this *** the present study,we modified the culture system for the induced differentiation from ESCs or EBs to PGCs,then the haploid gametes via *** to conventional maintenance and differentiation conditions basically using serum and feeder cells,we devoted to introduce a thoroughly serum-free and feeder-free system as monolayer and 3-D culture *** two different systems,we prepared ESC clones adhered to the dishes and suspended EB spheres at modest size ***-defined medium containing definite cytokines were used for *** on this serum-free and feeder-free culture system,we paid close attention to the spermatogenesis procedure at pivotal points, such as PGC initiation,transformation across meiosis,even the maturation of elongated sperm cells responding to each chemical-defined *** addition,we tracked out the expression profile and communication networks of hallmark genes and proteins represented typical features of different developmental stages of *** results provide an important cue for understanding the contributing molecular networks in the progress of spermatogenesis,and stimulate further research in this area as well. Conclusions:Our data showed that ESCs or EBs could be maintained and induced to form