Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells
作者单位：The Institute of Biomedical SciencesCollege of Life SciencesEast China Normal UniversityShanghaiChina Stem Cells and Regenerative Medicine CenterBaylor College of MedicineOne Baylor PlazaHoustonTexas 77030USA
会议名称：《第三届细胞结构与功能的信号基础研讨会》
会议日期：2010年
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
摘 要：Nanog and Oct4 are essential transcription factors that regulate self-renewal and pluripotency of ES ***,the mechanisms by which Nanog and Oct4 modulate ES cell fate remain *** characterization of endogenous Nanog and Oct4 protein complexes in mouse ES cells,we found that these transcription factors interact with each other and associate with proteins from multiple repression complexes,including the NuRD,Sin3A and Pml *** addition, Nanog,Oct4 and repressor proteins co-occupy Nanog-target genes in mouse ES cells, suggesting that Nanog and Oct4 together may communicate with distinct repression complexes to control gene *** our surprise,of the various core components in the NuRD complex with which Nanog and Oct4 interact,Mta1 was preferred,whereas Mbd3 and Rbbp7 were either absent or present at sub-stoichiometric *** named this unique Hdacl/2- and Mtal/2-containing complex NODE(for Nanog and Oct4 associated deacetylase).Interestingly,NODE contained histone deacetylase(HDAC) activity that seemed to be comparable to NuRD,and retained its association with Nanog and Oct4 in Mbd3-/- ES *** contrast to Mbd3 loss-of-function,knockdown of NODE subunits led to increased expression of developmentally regulated genes and ES-cell *** data collectively suggest that Nanog and Oct4 associate with unique repressor complexes on their target genes to control ES cell fate.